啤酒花HPLC指纹图谱研究
投稿时间:2018-05-21  修订日期:2018-10-08  点此下载全文
引用本文:董志敏,许月明,闻俊,蒋益萍,秦路平,辛海量.啤酒花HPLC指纹图谱研究[J].药学实践杂志,2019,37(1):42~45,54
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作者单位E-mail
董志敏 上海师范大学生命与环境科学学院, 上海 200234
海军军医大学药学院, 上海 200433 
 
许月明 海军军医大学药学院, 上海 200433  
闻俊 海军军医大学药学院, 上海 200433  
蒋益萍 海军军医大学药学院, 上海 200433  
秦路平 上海师范大学生命与环境科学学院, 上海 200234
海军军医大学药学院, 上海 200433
浙江中医药大学药学院, 浙江 杭州 310053 
qinsmmu@126.com 
辛海量 海军军医大学药学院, 上海 200433 hailiangxin@163.com 
基金项目:国家自然科学基金(U1603283)
中文摘要:目的 建立啤酒花HPLC指纹图谱。方法 色谱柱:Diamonsil C18柱(250 mm×4.6 mm,5 μm),流动相:乙腈-0.1%磷酸水,梯度洗脱,检测波长358 nm,柱压10.2 MPa,柱温30℃,进样量10 μl,流速1.0 ml/min。结果 首次建立啤酒花HPLC指纹图谱共有模式,对其19个共有峰进行标定,并对其中的黄腐酚、类葎草酮、葎草酮、加葎草酮、类蛇麻酮、蛇麻酮、加蛇麻酮进行指认。26批药材中有5批药材相似度低于或等于0.9,而该5批均为野生品种。结论 该方法高速有效,可准确地对啤酒花栽培种、野生种进行区分,为啤酒花的品质评价研究奠定基础。
中文关键词:啤酒花  高效液相色谱  指纹图谱
 
Study on fingerprint of Hupulus lupulus by HPLC
Abstract:Objective To establish fingerprint of Hupulus lupulus by HPLC. Methods Diamonsil C18 column (250 mm×4.6 mm, 5 μm) was used. The mobile phase consisted of acetonitrile and 0.1% phosphoric acid, in gradient elution mode, detection wavelength was 358 nm, column pressure at 10.2MPa, column temperature was 30℃, injection volume of 10 μl and flow rate was 1.0 ml/min. Results A common mode of HPLC fingerprint of Hupulus lupulus was established. 19 common peaks were calibrated, xanthohumol, cohumulone, humulone, adhumulone, colupulone, lupulone and adlupulone for identification. Among the 26 batches of Hupulus lupulus, 5 batches of herbs had similarities of less than or equal 0.9, which were all wild varieties. Conclusion The method was fast, effective, accurate and reliably to distinguish the cultivation and wildness Hupulus lupulus, which provided reference for quality control of Hupulus lupulus.
keywords:Hupulus lupulus  HPLC  fingerprint
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