玉屏风多糖组分对皮肤角质形成细胞的作用研究
投稿时间:2018-06-26  修订日期:2018-11-29  点此下载全文
引用本文:黄瑾,刘景雪,曾颖.玉屏风多糖组分对皮肤角质形成细胞的作用研究[J].药学实践杂志,2019,37(1):51~54
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作者单位
黄瑾 上海中医药大学附属岳阳中西医结合医院药学部, 上海 200437
上海市浦东新区人民医院药剂科, 上海 201299 
刘景雪 上海中医药大学附属岳阳中西医结合医院药学部, 上海 200437 
曾颖 上海市浦东新区人民医院药剂科, 上海 201299 
基金项目:上海市卫生和计划生育委员会科研课题资助项目(201440461);浦东新区卫生系统学科带头人培养计划(PWRd-2014-11);浦东新区卫生(计生)科技项目(PW2013A-12)
中文摘要:目的 研究中药方剂玉屏风的多糖组分在体外对角质形成细胞的免疫调节作用。方法 利用Real-time PCR法检测玉屏风多糖组分对激活的皮肤角质形成细胞中各炎症因子表达的调控作用;分别用Real-time PCR法和Elisa法对聚角蛋白微丝蛋白(Filaggrin)在mRNA和蛋白水平的表达进行检测;用Western bolt法检测多糖组分对NF-κB信号通路的调节作用。结果 玉屏风多糖组分明显抑制激活的皮肤角质形成细胞分泌炎症因子,促进Filaggrin的表达,并对NF-κB信号通路有明显的抑制作用,该信号通路对炎症和免疫细胞的活化有重要作用。结论 玉屏风多糖组分在体外对皮肤角质形成细胞有免疫调节作用,能够减缓皮肤局部炎症的发展,为玉屏风方剂的进一步开发应用奠定理论基础。
中文关键词:玉屏风  多糖  皮肤角质形成细胞  炎症因子
 
Study of the effect of polysaccharides from Yupingfeng on keratinocytes
Abstract:Objective To study the immunomodulatory effect of Yupingfeng polysaccharides on keratinocytes in vitro.Methods The effects of Yupingfeng polysaccharides on the expression of various inflammatory factors in the activated keratinocytes were detected by the Real-time PCR method.The mRNA and protein expression levels of Filaggrin were detected by Real-time PCR and Elisa respectively.The western bolt assay was used to detect the regulation of NF-κB signaling pathway by polysaccharides.Results Yupingfeng polysaccharides inhibited the secretion of inflammatory factors in the activated keratinocytes, promoted the expression of Filaggrin and exhibited significant inhibitory effect on the NF-κB signaling pathway, which plays an important role in the inflammation and immune response.Conclusion Yupingfeng polysaccharides had an immune regulation effect on skin keratinocytes in vitro and alleviated the development of local inflammation.These results lay a theoretical foundation for the clinical application of Yupingfeng prescription.
keywords:Yupingfeng  polysaccharide  keratinocytes  inflammatory factors
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