正极性驻极体5-氟尿嘧啶贴剂对兔耳瘢痕组织中Ⅰ、Ⅲ型胶原和TCF-β表达的影响
投稿时间:2018-06-15  修订日期:2018-11-12  点此下载全文
引用本文:安晓强,苑旺,梁媛媛,郭鑫,梁合鹃,江键,崔黎丽.正极性驻极体5-氟尿嘧啶贴剂对兔耳瘢痕组织中Ⅰ、Ⅲ型胶原和TCF-β表达的影响[J].药学实践杂志,2019,37(2):115~120,145
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作者单位E-mail
安晓强 海军军医大学药学院无机化学教研室, 上海 200433  
苑旺 海军军医大学卫勤系数理教研室, 上海 200433  
梁媛媛 海军军医大学卫勤系数理教研室, 上海 200433  
郭鑫 海军军医大学卫勤系数理教研室, 上海 200433  
梁合鹃 海军军医大学卫勤系数理教研室, 上海 200433  
江键 海军军医大学卫勤系数理教研室, 上海 200433  
崔黎丽 海军军医大学药学院无机化学教研室, 上海 200433 cuilili39@hotmail.com 
基金项目:国家自然科学基金面上项目(51477175)
中文摘要:目的 研究+5 kV驻极体、5-氟尿嘧啶(5-FU)贴剂和+5 kV驻极体5-FU贴剂对兔耳瘢痕组织中Ⅰ、Ⅲ型胶原和转化生长因子-β(TGF-β)表达的影响。方法 将通过电晕充电系统制备的正极性聚丙烯驻极体充电面与制备的5-FU贴剂背衬层结合制成表面电位为+5 kV的驻极体5-FU贴剂,再将+5 kV驻极体、5-FU贴剂和+5 kV驻极体5-FU贴剂分别作用于兔耳创面,通过测算瘢痕增生指数,观察研究瘢痕增生状况,通过HE染色、Masson染色和免疫组织化学的方法,研究上述驻极体、5-FU贴剂和驻极体5-FU贴剂对兔耳瘢痕组织中Ⅰ、Ⅲ型胶原和TGF-β表达的影响。结果 ①兔耳创面自然愈合4周后形成增生性瘢痕组织。②正极性驻极体不会导致创面过度修复。③5-FU贴剂和正极性驻极体5-FU贴剂通过减少瘢痕组织内Ⅰ型胶原、Ⅲ型胶原和TGF-β的表达量,抑制增生性瘢痕的生长。④正极性驻极体5-FU贴剂抑制增生性瘢痕生长的效果优于5-FU贴剂。结论 正极性驻极体与5-FU联用抑制增生性瘢痕生长的效果更好。
中文关键词:驻极体  增生性瘢痕  5-氟尿嘧啶  贴剂  Ⅰ型胶原  Ⅲ型胶原  转化生长因子-β
 
The effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue
Abstract:Objective To study the effects of positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagens and TGF-β in rabbit ear scar tissue.Methods The positive electret 5-fluorouracil (5-FU) patches with surface potential of+5 kV were made by attaching the positive charged polypropylene (PP) electret on the back layer of the 5-FU patch.The positive electrets,5-FU patch and positive electret 5-FU patches were applied to the wound surface on rabbit ear.The scar proliferation index was calculated to study the status of the scar.the HE staining,Masson staining and immunohistochemical methods were used to study the effects of the positive electrets,5-FU patch and the positive electret 5-FU patch on the expression of type Ⅰ,Ⅲ collagen and TGF-β in the rabbit ear scar tissue.Results ① The hypertrophic scar (HS) was formed after 4 weeks natural healing.② The positive electret will not lead to excessive wound repair.③ Both the 5-FU patch and the positive electret 5-FU patch reduced the expression of type Ⅰ collagen,type Ⅲ collagen and TGF-β in scar tissue and inhibited the growth of HS.④ The positive electret 5-FU patch had better inhibiting effect in HS growth than 5-FU patch.Conclusion The combination of positive electret and 5-FU can achieve better results in inhibiting the growth of hypertrophic scar.
keywords:electrets  hypertrophic scar  5-fluorouracil  patch  type Ⅰ collagen  type Ⅲ collagen  TGF-β
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