慢性不可预见性温和应激致抑郁对大鼠肝脏转运多肽mRNA和蛋白表达的影响
投稿时间:2018-06-11  修订日期:2018-10-15  点此下载全文
引用本文:刘萃萃,肖志军,陆赛花,徐峰.慢性不可预见性温和应激致抑郁对大鼠肝脏转运多肽mRNA和蛋白表达的影响[J].药学实践杂志,2019,37(2):121~125
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刘萃萃 上海健康医学院附属第六人民医院南院, 上海 201499  
肖志军 上海健康医学院附属第六人民医院南院, 上海 201499  
陆赛花 上海交通大学附属第六人民医院南院, 上海 201499  
徐峰 上海交通大学附属第六人民医院南院, 上海 201499 andrewfxu@sina.com 
基金项目:上海健康医学院种子基金项目(HMSF-16-22-023,SFP-18-20-15-002);上海奉贤区科委科学技术发展基金(20161008);上海市卫计委科研课题(20154Y0123)
中文摘要:目的 研究慢性不可预见性温和应激(chronic unpredicted mild stress,CUMS)致抑郁对大鼠肝脏有机阴离子转运多肽(organic anion transporting polypeptides,Oatps) mRNA和蛋白表达的影响。方法 24只雄性SD大鼠随机分为抑郁模型组和对照组,模型组连续8周给予CUMS,对照组正常饲养。分别于造模前后称量体重、进行糖水偏好实验和旷场实验,并测定大鼠血浆去甲肾上腺素和皮质酮水平以验证抑郁模型的建立。采用实时荧光定量聚合酶链式反应(RT-qPCR)和蛋白质印迹法(Western blotting)分别检测大鼠肝脏Oatp1a1、Oatp1a4和Oatp1b2 mRNA和蛋白表达。结果 与对照组相比,模型组大鼠体重减轻,糖水偏好百分比下降,垂直运动得分和水平运动得分减少,血浆去甲肾上腺素和皮质酮水平升高,肝脏Oatp1a1、Oatp1a4和Oatp1b2 mRNA和蛋白表达下降。结论 CUMS致抑郁下调大鼠肝脏Oatps mRNA和蛋白表达。
中文关键词:慢性不可预见性温和应激  抑郁  有机阴离子转运多肽
 
Effects of chronic unpredicted mild stress induced depression on mRNA and protein expression of liver organic anion transporting polypeptides in rats
Abstract:Objective To explore the impact of chronic unpredicted mild stress (CUMS)induced depression on mRNA and protein expression of liver organic anion transporting polypeptides (Oatps) in rats.Methods Twenty-four male SD rats were divided into depression group and control group.Rats in depression group were exposed CUMS for 8 weeks consecutively.Weight,open-field test,sucrose preference,plasma concentration of noradrenaline and corticosterone were assayed before and after model establishment to evaluate the depression.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were measured by RT-qPCR and Western blotting,respectively.Results In CUMS induced depression group,weight,sucrose consumption,vertical score and horizontal score were decreased while levels of corticosterone and norepinephrine were increased compared with control group.The mRNA and protein expression of liver Oatp1a1,Oatp1a4 and Oatp1b2 were reduced compared with control group.Conclusion The mRNA and protein expression of liver Oatps in rats were regulated down by CUMS induced depression.
keywords:chronic unpredicted mild stress  depression  organic anion transporting polypeptides
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