二氯乙酸钠对氧糖剥夺损伤的BV2细胞的保护作用及其机制研究
投稿时间:2018-10-20  修订日期:2018-12-27  点此下载全文
引用本文:赵辉,章越凡,李铁军.二氯乙酸钠对氧糖剥夺损伤的BV2细胞的保护作用及其机制研究[J].药学实践杂志,2019,37(2):146~150
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作者单位E-mail
赵辉 安徽中医药大学药学院, 安徽 合肥 230012  
章越凡 海军军医大学药学院药理学教研室, 上海 200433  
李铁军 安徽中医药大学药学院, 安徽 合肥 230012
上海市浦东新区浦南医院药剂科, 上海 200125 
18930502906@163.com 
基金项目:浦东新区科技发展基金(PKJ2015-Y26);浦东新区卫生和计划生育委员会学科建设项目(PWZbr 2017-16)
中文摘要:目的 研究二氯乙酸钠(dichloroacetate,DCA)对氧糖剥夺(oxygen glucose deprivation,OGD)损伤模型中小鼠小胶质细胞(BV2细胞)的保护作用,并探讨其作用机制。方法 将BV2细胞分为3组:对照组、OGD组、DCA治疗组,通过OGD 4 h建立损伤模型。CCK-8和流式细胞仪检测细胞凋亡及ROS和NO的表达,Western blot检测NF-κB通路相关蛋白表达水平。结果 CCK-8及流式细胞检测结果表明,DCA可显著降低OGD诱导的BV2细胞凋亡,并且减少OGD后细胞中活性氧(ROS)和一氧化氮(NO)的表达(P<0.05)。Western blot结果显示,DCA可显著影响OGD损伤介导的BV2细胞JNK、I-κB和NF-κB蛋白表达水平(P<0.05)。结论 DCA对OGD损伤的BV2细胞具有保护作用,其机制与抗凋亡、抗氧化和抗炎作用有关。
中文关键词:二氯乙酸钠  氧糖剥夺  ROS  NF-κB
 
Protective effect and mechanism of sodium dichloroacetate on BV2 cells damaged by OGD
Abstract:Objective To study the protective effect and mechanism of dichloroacetate (DCA) on BV2 microglia in oxygen glucose deprivation (OGD) injury model.Methods BV2 cells were divided into 3 groups:CON group,OGD group and DCA intervention group.The injury model was established by OGD 4 h.CCK-8 and flow cytometry was used to detect apoptosis and expression of ROS and NO in cells.Western blot was used to detect the expression of proteins related to NF-κB pathway.Results CCK-8 and flow cytometry showed that the apoptosis of BV2 cells induced by OGD and the expression of ROS and NO in OGD cells were decreased significantly by DCA (P<0.05).Western blot analysis showed that the expression of I-κB,JNK and NF-κB protein in BV2 cells mediated by OGD injury were influenced significantly by DCA (P<0.05).Conclusion DCA had a protective effect on OGD-injured BV2 cells through anti-oxidation and anti-apoptosis.
keywords:dichloroacetate  OGD  ROS  NF-κB
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