HPLC法同时测定复方金银花颗粒中10种成分的含量
投稿时间:2018-07-25  修订日期:2018-11-22  点此下载全文
引用本文:何悦,王四旺,肖会敏.HPLC法同时测定复方金银花颗粒中10种成分的含量[J].药学实践杂志,2019,37(2):177~182
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作者单位E-mail
何悦 陕西康城药业股份有限公司, 陕西 商洛 726000
空军军医大学中药和天然药物学教研室, 陕西 西安 710032 
 
王四旺 空军军医大学中药和天然药物学教研室, 陕西 西安 710032  
肖会敏 空军军医大学中药和天然药物学教研室, 陕西 西安 710032
陕西含光生物科技有限公司, 陕西 西安 710032 
xhm_0908@163.com 
中文摘要:目的 建立HPLC法同时测定复方金银花颗粒中10种成分(新绿原酸、绿原酸、隐绿原酸、咖啡酸、连翘酯苷B、连翘酯苷A、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素)含量的方法。方法 采用HPLC法,色谱柱:Kromasil C18(250 mm×4.6 mm,5 μm);流动相:乙腈-0.1%磷酸水溶液,梯度洗脱;流速:0.8 ml/min;检测波长:320 nm;柱温:35℃;进样量:10 μl。结果 在上述色谱条件下,10个成分的峰具有良好的分离度;新绿原酸、绿原酸、隐绿原酸、咖啡酸、连翘酯苷B、连翘酯苷A、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的线性范围分别为10.20~340.06、7.19~239.81、6.12~203.84、15.00~500.00、20.16~671.85、25.19~839.67、14.61~487.14、10.52~350.66、7.32~244.01、7.61~253.82 μg/ml;10个对照品的平均加样回收率均在97.67%~99.00%之间,RSD均在0.94%~1.37%之间;10批制剂中新绿原酸、绿原酸、隐绿原酸、咖啡酸、连翘酯苷B、连翘酯苷A、黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的平均含量分别为0.579 0、1.523 5、1.080 9、0.083 1、0.468 0、0.739 6、1.977 9、0.351 5、0.246 8、0.256 6 mg/g。结论 该含量测定方法简便、稳定可靠、准确,可作为复方金银花颗粒质量评价的方法。
中文关键词:复方金银花颗粒  高效液相色谱  新绿原酸  隐绿原酸  绿原酸  连翘酯苷A  连翘酯苷B  黄芩苷
 
Simultaneous assay of 10 active components in compound Jinyinhua granules by HPLC
Abstract:Objective To establish an one step assay method for 10 active components (neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin)in compound Jinyinhua granules.Methods The Kromasil C18 column (250 mm×4.6 mm,5 μm) was used with the column temperature at 35 ℃ and PDA detection wavelength at 320 nm.A gradient elution with acetonitri-0.1% phosphoric acid was adopted at the flow rate of 0.8 ml/min.The injection volume was 10 μl.Results The good resolution was achieved for the 10 active components.The linear ranges of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin were 10.20-340.06,7.19-239.81,6.12-203.84,15.00-500.00,20.16-671.85,25.19-839.67,14.61-487.14,10.52-350.66,7.32-244.01,7.61-253.82 μg/ml respectively.The average recoveries (n=6) were between 97.67% and 99.00% and RSDs between 0.94% and 1.37%.The average contents of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,forsythoside B,forsythoside A,baicalin,wogonoside,baicalein,wogonin from 10 batches of preparations were 0.579 0,1.523 5,1.080 9,0.083 1,0.468 0,0.739 6,1.977 9,0.351 5,0.246 8 and 0.256 6 mg/g.Conclusion This simple and accurate method can be used as one of quality control methods for the compound Jinyinhua granules.
keywords:compound Jinyinhua granule  HPLC  neochlorogenic acid  cryptochlorogenic acid  chlorogenic acid  forsythoside A  forsythoside B  baicalin
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