绿萼梅总黄酮对慢性温和刺激所致抑郁大鼠神经内分泌和氧化应激的影响
投稿时间:2018-06-28  修订日期:2019-01-20  点此下载全文
引用本文:陈静,陈明珠,黄玉香,程晶,黄雅平.绿萼梅总黄酮对慢性温和刺激所致抑郁大鼠神经内分泌和氧化应激的影响[J].药学实践杂志,2019,37(3):226~230
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作者单位E-mail
陈静 泉州医学高等专科学校药理学教研室, 福建 泉州 362000  
陈明珠 泉州医学高等专科学校药理学教研室, 福建 泉州 362000  
黄玉香 泉州医学高等专科学校药理学教研室, 福建 泉州 362000 63885519@qq.com 
程晶 泉州医学高等专科学校药理学教研室, 福建 泉州 362000  
黄雅平 泉州医学高等专科学校药理学教研室, 福建 泉州 362000  
基金项目:福建省中青年教师教育科研项目(JAT160848)
中文摘要:目的 通过观察绿萼梅总黄酮(TFAM)对慢性温和刺激抑郁大鼠神经内分泌及氧化应激的影响,探讨其可能的抗抑郁机制。方法 将SD大鼠随机分为6组:生理盐水组、模型组、阳性对照组(氟西汀,20 mg/kg)、低剂量治疗组(TD-TFAM,80 mg/kg)、中剂量治疗组(TZ-TFAM,160 mg/kg)和高剂量治疗组(TG-TFAM,240 mg/kg);除生理盐水组外,其余各组大鼠经历28 d慢性不可预见性温和应激(CUMS)造模成功后随机分组。通过大鼠体重增量变化、糖水偏好实验、旷场实验评估TFAM的抗抑郁作用;采用酶联免疫吸附法(Elisa)测定大鼠海马和前额叶皮层去甲肾上腺素(NA)、5-羟色胺(5-HT)、促肾上腺皮质激素(ACTH)和皮质酮(CORT)含量,评估TFAM对神经递质表达量的影响;通过检测大鼠血清和纹状体中谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)的活性评估TFAM对氧化应激的影响。结果 TFAM能显著增加CUMS大鼠体重增值变量,提高糖水偏爱百分比,逆转旷场实验评分,提高大鼠海马和前额叶皮层NA、5-HT的含量,降低ACTH、CORT的含量,增强大鼠血清和纹状体中GSH-Px和SOD的活性。结论 TFAM抗抑郁作用可能与促进单胺递质分泌、调节下丘脑-垂体-肾上腺(HPA)轴功能和清除体内自由基有关。
中文关键词:绿萼梅总黄酮  抗抑郁  单胺神经递质  下丘脑-垂体-肾上腺轴  抗氧化
 
Effects of total flavonoids from Armeniaca mume on neuroendocrine and oxidative stress of rats with depression induced by chronic mild stress
Abstract:Objective To explore the possible mechanism of total flavonoids from Armeniaca mume(TFAM) through observing its effects on neuroendocrine and oxidative stress of rats with depression induced by chronic mild stress.Methods The SD rats were randomly divided into normal saline group,model group,positive control group (fluoxetine,20 mg/kg),low-dose treatment group (TD-TFAM,80 mg/kg),middle-dose treatment group (TZ-TFAM,160 mg/kg) and high-dose treatment group (TG-TFAM,240 mg/kg),total 6 groups.Except the normal saline group,the rats in all other groups were given chronic unpredictable mild stress (CUMS) for 28 d to build the depression models,and then were randomly assigned.The antidepressant effects were evaluated by index such as growth in weight,sucrose preference and open-field test.Its effects on neuroendocrine were evaluated by contents of noradrenaline (NA),5-hydroxytryptamine (5-HT),adrenocorticotropic hormone (ACTH) and corticosterone (CORT) in the hippocampus and prefrontal cortex through enzyme linked immunosorbent assay (ELISA). Also,the enzymes activities of glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in the serum and striatum were measured to estimate its influences on oxidative stress.Results TFAM significantly increased the weight growth of CUMS rats,promoted the percentage of sugar water preference,reversed open-field test score,increased the contents of 5-HT and NA but reduced the contents of ACTH and CORT in the hippocampus and prefrontal cortex of rats,while the activities of SOD and GSH-Px in serum and striatum were up-regulated.Conclusion The anti-depressant mechanism of TFAM might be related to promoting the secretion of monoamines,regulating the function of hypothalamic-pituitary-adrenal (HPA) axis and removing free radicals in rats.
keywords:total flavonoids from Armeniaca mume  depression  monoamine neurotransmitter  hypothalamic-pituitary-adrenal  HPA  antioxidant
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