升陷汤及各单味药对阿霉素致心肌细胞损伤的保护作用
投稿时间:2018-09-05  修订日期:2019-02-26  点此下载全文
引用本文:满缓,张凤,黄豆豆,熊筱娟,詹勤,廖丽娜,李铁军,陈万生.升陷汤及各单味药对阿霉素致心肌细胞损伤的保护作用[J].药学实践杂志,2019,37(4):304~308
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满缓 海军军医大学附属长征医院药材科, 上海 200003
宜春学院化学与生物工程学院, 江西 宜春 336000 
 
张凤 海军军医大学附属长征医院药材科, 上海 200003  
黄豆豆 海军军医大学附属长征医院药材科, 上海 200003  
熊筱娟 宜春学院化学与生物工程学院, 江西 宜春 336000  
詹勤 海军军医大学附属长征医院药材科, 上海 200003  
廖丽娜 海军军医大学附属长征医院药材科, 上海 200003  
李铁军 海军军医大学药学院药理教研室, 上海 200433  
陈万生 海军军医大学附属长征医院药材科, 上海 200003 chenwansheng@smmu.edu.cn 
基金项目:国家自然科学基金(81470173),上海市科技成果转化和产业化项目(18401931600)
中文摘要:目的 研究升陷汤(SXT)及各单味药对阿霉素致心肌细胞损伤的保护作用。方法 将乳鼠缺氧/复氧培养的心肌细胞分为正常对照组(Con组)、阿霉素诱导损伤组(M组)和不同药物(升陷汤及其各单味药)治疗阿霉素诱导损伤组(SXT、A-E组)。检测指标包括细胞活力、细胞活性氧族(ROS)、Ca2+和乳酸脱氢酶(LDH)水平。结果 与正常组相比,阿霉素能够造成心肌细胞损伤,与模型组比较,升陷汤全方以及各单味药实验组均能增强细胞的活力,降低细胞Ca2+和LDH浓度;除桔梗和升麻外,升陷汤全方及其他单味药能够降低ROS的浓度,实验结果提示,在早期阿霉素治疗中如果应用升陷汤,一定程度上能降低阿霉素的心脏毒性作用。结论 升陷汤及各单味药对阿霉素致心肌细胞损伤具有保护作用。
中文关键词:升陷汤  阿霉素  心肌细胞损伤  保护作用
 
Protective effect of Shengxian decoction and each single herb component against adriamycin-induced cardiomyocyte injury
Abstract:Objective To study the protective effect of Shengxian decoction (SXT) and its each single herb component against doxorubicin induced cardiomyocyte injury.Methods Myocardial cells isolated from the neonatal rats with hypoxia/reoxygenation injury of cardiomyocytes were divided into eight groups,control group (Con group),doxorubicin-induced injury group (M group),and treatment groups with Shengxian decoction and its each single herb decoction (SXT,A-E groups) following doxorubicin-induced injury.The indicators tested included cell viability,cell ROS,Ca2+ and LDH levels.Results Compared with the control group,doxorubicin induced obvious myocardial injury in model group.Compared with the model group,all tested drug groups increased the cell viability,reduced the levels of cell Ca2+ and LDH.Besides,SXT and the single herb decoction reduced the concentration of ROS except the decoction of Platycodon grandiflorum and Cimicifuage Rhizoma.The experimental results suggested that the application of SXT in the early episode could reduce the cardiac toxicity of adriamycin to a certain extent.Conclusion SXT and its each single herb decoction showed protective effect against doxorubicin-induced cardiomyocyte injury.
keywords:Shengxian decoction  adriamycin  cardiomyocyte injury  protection effect
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