基于GC-MS技术的金钱草干预结晶肾损伤小鼠血清代谢组学研究
投稿时间:2018-12-13  修订日期:2019-05-07  点此下载全文
引用本文:杜毅,丁德英,吕磊,李玲.基于GC-MS技术的金钱草干预结晶肾损伤小鼠血清代谢组学研究[J].药学实践杂志,2019,37(4):332~336
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作者单位E-mail
杜毅 上海交通大学医学院附属新华医院药学部, 上海 200092  
丁德英 海军军医大学药学院分析测试中心, 上海 200433  
吕磊 上海东方肝胆外科医院药材科, 上海 200438  
李玲 海军军医大学药学院分析测试中心, 上海 200433 316262150@163.com 
中文摘要:目的 利用血清代谢组学方法监测结晶肾损伤相关的代谢变化,评价金钱草提取物治疗结晶肾损伤的作用。方法 将21只野生型C57BL/6小鼠随机分为3组:空白对照组、结晶肾损伤模型组和金钱草干预组。采用乙醛酸盐诱导小鼠结晶肾损伤模型,给予6 d相应的治疗后,取小鼠血清进行气相色谱-质谱联用仪分析,结合模式识别筛选模型组和对照组之间的差异代谢物,并以此评价金钱草的治疗作用。结果 血清代谢组学分析鉴定了14种结晶肾损伤相关的生物标志物,包括嘌呤代谢物、氨基酸代谢物、脂肪酸代谢物等,同时数据显示金钱草提取物对结晶肾损伤有改善作用。结论 金钱草提取物能够通过调节失衡的嘌呤代谢、氨基酸代谢、糖代谢等途径而发挥预防和治疗结晶肾损伤作用。
中文关键词:结晶肾损伤  金钱草  气相色谱-质谱联用  代谢组学
 
Metabolomics study of Lysimachiae Herba on mice with crystallization kidney injury by GC-MS
Abstract:Objective To evaluate therapeutic effects of Lysimachiae Herba for crystallization kidney injury with serum metabolomics method.Methods 21 rats were randomly divided into three groups including control group,crystallization kidney injury model group and Lysimachiae Herba-treated group.The medicated group received Lysimachiae Herba for 6 days.Then,the serum samples of each rat were collected and analyzed by Gas Chromatography-Mass Spectrometer (GC-MS).The pattern recognition analysis of metabolomics differences among three groups was used to evaluate therapeutic effects of Lysimachiae Herba.Results 14 metabolites were identified through serum metabolomics analysis.The possible mechanism of crystallization kidney injury was mainly related to the metabolic products of purine,amino acid,fatty acid and sugar.The kidney injury by crystallization was alleviated by Lysimachiae Herba.Conclusion Lysimachiae Herba has preventive and therapeutic effects for crystallization kidney injury through partially regulating the perturbed purine metabolism,amino acid metabolism and glycometabolism.
keywords:kidney injury by crystallization  Lysimachiae Herba  GC-MS  metabolomics
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