基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究
投稿时间:2019-03-15  修订日期:2019-05-22  点此下载全文
引用本文:曾棋平,蔡小辉,吴坤林,杨丽娜,曹毅祥,陈锦珊.基于正交投影偏最小二乘法对复方首乌藤合剂抗失眠作用的谱效关系研究[J].药学实践杂志,2019,37(5):427~432
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曾棋平 中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科, 福建 漳州, 363000  
蔡小辉 中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科, 福建 漳州, 363000  
吴坤林 中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科, 福建 漳州, 363000  
杨丽娜 中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科, 福建 漳州, 363000  
曹毅祥 中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科, 福建 漳州, 363000  
陈锦珊 中国人民解放军联勤保障部队第九○九医院/厦门大学附属东南医院制剂科, 福建 漳州, 363000 cjs18659341758@163.com 
基金项目:医院青年苗圃课题(16Y024);福建中医药大学临床专项校管课题(XB2017080)
中文摘要:目的 探讨复方首乌藤合剂抗失眠作用与HPLC指纹图谱间的相关性。方法 采用HPLC分析得到不同提取工艺条件下的复方首乌藤合剂指纹图谱;腹腔注射DL-4-氯苯丙氨酸建立大鼠失眠模型,用ELISA法测定大鼠海马体5-HT的含量,采用正交投影偏最小二乘法分析谱效关系。结果 从指纹图谱中共确定21个共有峰,12个峰的变量投影重要性(VIP)均>1.0,复方首乌藤合剂指纹图谱中3个色谱峰对抗失眠发挥重要作用。结论 复方首乌藤合剂抗失眠作用的物质基础与其所含多种成分共同作用的结果有关,通过谱效关系分析得到复方首乌藤合剂的抗失眠物质基础,可为制剂的质量控制提供实验依据。
中文关键词:复方首乌藤合剂  指纹图谱  抗失眠  谱效关系  正交投影偏最小二乘法
 
Study on spectrum-effect relationship of sedative and hypnotic effect of compound caulis polygoni multiflori mixture based on orthogonal partial least squares regression analysis
Abstract:Objective To investigate the correlation between anti-insomnia effects and HPLC fingerprint of compound caulis polygoni multiflori mixture.Methods A fingerprinting method was established by high performance liquid chromatography and then used to analyze different batches of compound caulis polygoni multiflori mixture.Rat insomnia model was established by intraperitoneal injection of p-chlorophenylalanine,5-HT in rat hippocampus was determined by ELISA.Then the orthogonal partial least squares (OPLS)analysis was used to investigate the spectrum-effect relationship.Results 21 common peaks were identified from the fingerprints,the variable projection importance (VIP)values of these 12 peaks were more than 1.0,indicated that the 3 peaks had played an important role in the anti-insomnia activity of compound caulis polygoni multiflori mixture.Conclusion The anti-insomnia activity of compound caulis polygoni multiflori mixture was the result of synergistic effect of multiple components,its material basis could be analyzed by spectrum-effect relationship,which could provide basis for the quality control of preparation.
keywords:compound caulis polygoni multiflori mixture  fingerprint  anti-insomnia  spectrum-effect relationship  orthogonal partial least squares
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